Compare and contrast the poems Essay Example for Free

Compare and contrast the poems Essay The poem leaves us either reassured that in the midst of all evil there can be some good or depressed and in despair because in the family life of the Belson commandant lurks an evil which could ruin their lives any time. Night of the Scorpion The title denotes power and control from the scorpion as one night the scorpion ruled and controlled everything that happened. The title uses Night to give a sense of dark times and that something fatal may occur. The poem is very other. It has an Indian location which is where scorpions can be found as it is one of the warmer regions of the world. The poem is in free verse with the last three lines sectioned off. It is of narrative style and contains a very memorable heart. The weather was desperate and it was lashing with rain. In fact the monsoon conditions had affected the scorpion and all that it wanted to do was to shelter from the torrent. However it was disturbed by the poets mother who was probably searching for rice to feed her family. She was stung and the poison from the tail entered her bloodstream like a foreign invader defiling the enemy territory. Many neighbours arrived and the author compares this to a swarm of flies. I would compare it to students who clamour around desperate fro entertainment around a schoolyard fight or would be helpers around an accident victim. The neighbours like the helpers all had their suggestions many of their ideas involving the power, rituals and beliefs of religion. The scorpion is thought to be evil when in reality it is only trying to protect itself. The people believed that the victim and the scorpion were still linked. This bond meant that when the scorpion moved the poison inside the mother moved around her blood invading and conquering. Many prayers were said for the victim. The neighbours also felt that out of this tragic accident some good would emerge. The poison would burn away her sins and cleanse and purify her body of excessive ambition and lustful or adulterous thoughts or acts. Neighbours believed that her suffering was paying the price of evil to God and reducing the amount of evil in the world. The victims husband was willing to try any one idea or a combination and mixture of the suggestions. He was usually not one to believe in religion but usually based his opinions on logic and facts. Yet even he set fire to the affected site and watched the flame feeding on her toe. A holy man was allowed to perform sacred rites and after twenty hours the victim recovers only to rejoice in the fact that it was her and not her children. The mothers wishes were a superb example of the unconditional love most mothers feel for their children. Comparisons and Differences. Both poems are about creatures who are simply concerned with their own survival. With the vultures it is the need for food that causes them to be scavenges and with the scorpion its wish not to be squashed causes it to flash its diabolic tail. Both poems give information on cultures that are not familiar to us. The main difference is the message given by the poets In vultures the readers can chose there own position with regards to good and evil whereas the second message denotes the helplessness that sometimes death will occur and at other times the victim will survive. In the scorpion it is possible for both the victim and the scorpion to survive whereas the vultures will die if they dont eat prey and the prey is already dead. Death is a necessary evil for the vultures. Cultural Background I think that Night of the Scorpion best captures cultural background because it mentions religion and family life from another culture. For example mud baked walls and candles and lanterns and the Holy man performing his rights to tame the poison with an incantation. Whereas vultures is set in the middle of nowhere, somewhere like the deserted plains of Africa. It isnt something that would be found happening in a village. Scorpion is a true story of the poets family and it comes across through the detail and building of atmosphere the poem contains brought about by the phrase ten hours of steady rain had driven him to crawl beneath a sack of rice. As he was there at the time his thoughts and feelings are brought across very well in his writing. The poem shows compassion when the mother only said Thank God the scorpion picked on me and spared my children. Conclusion I prefer vultures as I like the way in which evil is contained in good. This is shown in the phrase in the very germ of that kindred love is lodged the perpetuity of evil. The evil is described in phrases such as picked the eyes of a swollen corpse, ate the things in its bowel and fumes of human roast. The good however is conjured up by inclined affectionately and tender offspring. I feel it is more of a poem compared to Night of the Scorpion because Scorpion is a very much narrative style of writing. It is too narrative for my liking.

Enzyme Linked Immunosorbent Assay

Enzyme Linked Immunosorbent Assay IMMUNOLOGY PRATICAL ASSIGNMENT ENZYME LINKED IMMUNOSORBENT ASSAY mODULE NAME: Clinical immunology MODULE NUMBER: APS6004 MODULE LEADER: DR JULIA REY-NORES STUDENT NUMBER: 20031761 BSc (H) BMS 3 2014/2015 Introduction The history of immunoassay was developed by Roalyn Yalow and Solomon Berson in 1950 used the Radio-immunoassay (RIA) and they awarded in 1977 Nobel Prize because they developed RIA to detect and measure the level of glucose in the blood for diabetic patient. However, the technology was build up by replacing the radio-isotopes with enzymes to make colour generation that was in 1960. ]1, 2, 3[ More than 40 years, immunoassays use in different places, like laboratory medicine, hospitals and research to improve the health also for many purposes. In addition, immunoassay use in life science research to study the biology system by chase different, hormones, proteins and antibody. However, it use in industry to detect contaminants in food and water. Also, used as quality control to observe specific molecules used through product processing. ]1[ Nowadays, the immunochemistry technology develops assays to try eliminate as many dilution, mixing and measuring. Immunoassays are technique used to detect specific molecule. It’s rely on the ingrained ability of antibody that are bind to the specific structure of molecules. This techniques are quick and accurate it’s depend on the antibody and antigen that found in the blood and tissue fluids. ]1[ There are many type of immunoassays such as radioimmunoassay (RIA), enzyme immunoassay (EIA), fluorescent immunoassay (FIA), and enzyme-linked immunoassay (ELISA). ]3,5[ In this report file I will focus on ELISA this technique that I used in the laboratory during two weeks to detect the antibody and the antigen. There is different types of ELISA: Direct ELISA Indirect ELISA Sandwich ELISA Competitive ELISA [3] Direct ELISA This method technique depend on the antigen that coated in the surface of plate and the antibody of the patient and conjugated enzyme. ]5[ Figure 1 shows steps of direct ELISA The indirect ELISA The technique used the micro plate coated with antigen. The primary antibody added to react with the antigen that fixed to the plate. Then washed away. Added enzyme conjugated secondary antibody anti-isotope antibody which binds to the primary antibody. After that washed away and added the substrate enzyme to produce the reaction colour that determined the amount of the antibody. ]3,7[ Figure 2 show the steps of the indirect ELISA Sandwich ELISA Sandwich ELISA is the technique that used to detect the antibody or antigen that are present in the patient blood. This technique also called capture method because it detect level of antigen between two layers of antibodies. The antigen to be measure in the technique should contain at least two antigenic epitope capable of binding antibody. Sandwich ELISA has many advantages for example high specificity, flexibility and sensitivity. ]3,8[ Figure 3 shows the steps of sandwich ELISA The aim of practical: To achieve a grid experimental to detect the optimal detection and capture antibody titration, by using monoclonal mouse anti-rabbit IgG and polyclonal goat anti-rabbit IgG antibodies. To determine the concentration of unknown sample X and Y. Materials: Coating buffer: phosphate buffer saline (PBS) Wash buffer: 0.05% Tween 20 ®in PBS, pH 7.4 Diluent: PBS Blocking solution: 1% (w/v) bovine serum albumin (BSA) in PBS Antigen : rabbit IgG Coating antibody: Mouse anti-rabbit IgG monoclonal antibody Detection antibody :Goat anti-rabbit IgG – peroxidase conjugated Colour reagent .tetramethyl benzidine (TMB) Stop solution (1M HCL) 96-well micro plate Adjustable micropipette Practical week 1 Methods: This step was done by the lab technician to make the 96-well plate coated with antigen ready to the students because it’s take long time. Figure 4 show the rabbit IgG antigen serial dilution by using 100 µl coating buffer Monoclonal and polyclonal antibody procedure in tables 1 and 2: Procedure of Monoclonal antibody Procedure of Polyclonal antibody Added 100 µl of diluents of buffer PBS from column 2-6 in the first plate Added 100 µl of buffer PBS from column 8-12 in the second half of the plate Added 200 µl of monoclonal mouse anti-rabbit IgG from A1well to H1 well Added 200 µl of goat anti-rabbit IgG HRP to column 7 transferred 100 µl by doing serial dilution mixed well from column 1 to columns 2 ,3,4,5,6 then discard 100 µl from well 6 Transferred 100 µl by doing serial dilution from column 7 to columns 8,9,10,11,12 then 100 µl discard from colum12 (mixed well) Covered the plate and incubated at room temperature for 30 minutes. Covered the plate and incubated at room temperature for 30 minutes. Washed the plate three times with wash buffer Washed the plate three times with wash buffer Table 1 The final steps 100 µl of goat anti-mouse IgG-HRP was added to columns 1 to 6 200 µl of goat anti-rabbit IgG-HRP was added to columns 7 to 12 The plate was covered and incubated at room temperature for 30 minutes The plate was washed three times with the washing buffer 100 µl of substrate (TMP) was added to all the columns from 1-12 and incubated at room temperature and the plate was observed to check the change of colour to blue colour. After the colour become blue 50 µl of stop reaction 1 M HCL was added to all wells The colour will change to yellow The result was read by spectrophotometer Table 2 Figure 7 shows steps Week one: result Graph 1 shows the results of mouse anti-rabbit IgG monoclonal antibody titration in different dilution Result Graph 2 shows results of goat anti-rabbit IgG HRP labelled antibody in different dilution Practical week 2 Method: Step done by lab technician coated 20 wells overnight with 100 µl/well of the capture antibody (monoclonal mouse anti-rabbit IgG ) and kept ready for use.( sandwich ELISA) Added 200 µl of rabbit IgG to well A1 and A2 Added 100 µl of PBS diluents to wells from B to H in column 1and 2 From A1, 100 µl rabbit IgG was taken and added to B1 then serial dilution take place up to G1 then 100 µl was discarded from G1 100 µl of rabbit IgG was taken from A2 and added to B2 then continued the serial dilution up to G2 then 100 µl from G2 discarded Well H1 and H2 was used as blank Added 100 µl of unknown sample X to wells (A3 and A4) Added 100 µl of unknown sample Y to wells (B3 and B4) Incubated the plate for 30 minutes at room temperature Washed the plate 3 times with washing buffer PBS Added 100 µl of goat anti-rabbit IgG HRP labelled to all 20 wells Incubated the plate for 30 minutes at room temperature Washed the plate 3 times with Buffer BPS Added 100 µl TMB substrate to all 20 wells Incubated the plate and protected from the light until colour develops Added 50 µl stop reaction with (1 M HCL acid) Read absorbance at 450 nm by spectrophotometer The result: Graph 3 shows standard calibration curve of rabbit IgG Graph 4 shows the equation log of concentration rabbit IgG Calculation of samples Table 3 shows the calculation to found the concentration of samples X and Y Discussion From the result that shows in graph one there are six curves of the monoclonal mouse anti-rabbit IgG with different serious dilutions(1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000). From my result, the dilution 1:2000 is increase fast and it consumption more antibodies which is not recommended. The best dilution is 1:4000 because it gradually increase with less antibodies and this dilution can detect the lowest concentration of antigen and also can be used for more numbers of samples. However, the dilution 1:8000 it increase but is less than dilution 1:4000 it need more antibody, while the dilutions (1:16000, 1:32000, 1:64000) need more antibodies and not detect antigen in low concentration. The graph 2 shows the result of polyclonal antibody and the graph has sex different curves with different serious dilutions ((1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000) the first dilution 1:2000 increase sharp until concentration of 1000, then decrease slowly up concentration of 2000 so this dilution not recommended due to over opsonisation of antibodies. The second, dilution 1:4000 increased gradually and it need less antibody and can detect the lowest concentration of antigen so it is the optimum for the goat anti-rabbit IgG HRP labelled antibody. Third dilution 1:8000 is increase slow and require more antibody. The last three dilutions, 1:16000, 1:32000, and 1:64000 are not showing significant elevation when increase the concentration and cannot used because it not detect high absorbance of antigen. The graph 3 shows the calibration curve of the known concentration to determine the concentration of two unknown samples X and Y. the graph 4 shows the equation make by log concentration of calibration curve to calculated the concentration of unknown samples. During this practical I learned a lot of important things such as the best technique to choose the dilution of antibody and antigen detection. Also, to compare between the best antibody to detect antigen. There are many factors that affect the result of ELISA like the incubation time should be 1 hour but we reduced to 30 minutes and this not enough for the reaction take place between antibody and antigen, manual washing cause insufficient washing and mixed with other micro plate wells. The pipettes some time not working due to some problem of tips. Conclusion In conclusion, the optimum monoclonal Mouse anti-rabbit IgG antibody concentration is 1/4000, while the optimum polyclonal Goat anti-rabbit IgG HRP labelled antibody concentration is 1/4000, and the concentration of unknown sample( X )is 287ng/ml and unknown of sample (Y) concentration is 41ng/ml. the ELISA is the best technique to detect the reaction between antibody and antigen. Reference 1-Avrameas, S. (2006). Historical Background of the Invention of EIA and ELISA. Clinical Chemistry, 52(7), pp.1430a-1431. 2Tulsidas G, S. (2002). HISTORY AND DEVELOPMENT OF ANALYTICAL CHEMISTRY IN LIFE SCIENCES WITH REFERENCE TO IMMUNOASSAY IN MEDICINE. Health and Population, 3(25), pp.140-147. 3- Owen, J. et al. 2009. Immunology by Kuby. 7th ed. New York: W. H Freeman and Company. 4-Immunochemistry.com, (2014). Apoptosis, Caspases, Assay Development, ELISA Buffers, ELISA Detection. [online] Available at: http://www.immunochemistry.com [Accessed 26 Nov. 2014]. 5-Accelero-bioanalytics.com, (2014). Home Accelero ® Bioanalytics GmbH. [online] Available at: http://www.accelero-bioanalytics.com [Accessed 27 Nov. 2014] 6-Wieslab.com, (2014). Wieslab Laboratory Services Home. [online] Available at: http://www.wieslab.com [Accessed 27 Nov. 2014]. 7-Pharmatutor.org, (2014). Articles | PharmaTutor. [online] Available at: http://www.pharmatutor.org/articles [Accessed 27 Nov. 2014]. 8-Elisa-antibody.com, (2014). ELISA Antibody, protocol and troubleshooting. [online] Available at: http://www.elisa-antibody.com [Accessed 27 Nov. 2014]. Appendix Result week one practical 1 2 3 4 5 6 7 8 9 10 11 12 2.1104 2.1292 1.9613 1.6637 1.3974 1.2574 3.2528 1.8449 0.9561 0.4939 0.2465 0.1338 1.8208 1.5499 1.4053 1.5323 1.0412 0.7042 3.4643 1.5967 0.8303 0.4028 0.2565 0.1613 1.4231 1.3054 0.5794 0.9972 0.8248 0.6163 2.8907 1.313 0.6298 0.3189 0.1761 0.1112 1.0608 0.9475 0.8302 0.6554 0.5236 0.3168 2.2198 1.065 0.5392 0.2867 0.1652 0.1013 0.7257 0.7008 0.6846 0.6725 0.5747 0.5967 1.6108 0.7602 0.6945 0.3432 0.1921 0.1128 0.513 0.4868 0.4624 0.3917 0.4104 0.3967 0.9931 0.5756 0.3218 0.17 0.1043 0.1606 0.3335 0.3444 0.3188 0.3414 0.3042 0.2611 0.6909 0.3377 0.1896 0.1087 0.0786 0.0585 0.0797 0.0856 0.0774 0.0677 0.0772 0.0886 0.1005 0.0566 0.0459 0.0473 0.0498 0.0589 Table 1 shows the result of the absorbance of monoclonal antibody and polyclonal antibody concentration 1/2000 1/4000 1/8000 1/16000 1/32000 1/64000 2000 2.0307 2.0436 1.8839 1.596 1.3202 1.1688 1000 1.7411 1.4643 1.3279 1.4646 0.964 0.6156 500 1.3434 1.2198 0.502 0.9295 0.7476 0.5277 250 0.9811 0.8619 0.7528 0.5877 0.4464 0.2282 125 0.646 0.6152 0.6072 0.6048 0.4975 0.5081 62 0.4333 0.4012 0.385 0.324 0.3332 0.3081 31 0.2538 0.2588 0.2414 0.2737 0.227 0.1725 0 0 0 0 0 0 0 Table 2 the results of the absorbance of monoclonal antibody after subscription of the absorbance from blank concentration 1/2000 1/4000 1/8000 1/16000 1/32000 1/64000 2000 3.1523 1.7883 0.9102 0.4466 0.1967 0.0749 1000 3.3638 1.5401 0.7844 0.3555 0.2067 0.1024 500 2.7902 1.2564 0.5839 0.2716 0.1263 0.0523 250 2.1193 1.0084 0.4933 0.2394 0.1154 0.0424 125 1.5103 0.7036 0.6486 0.2959 0.1423 0.0539 62 0.8926 0.519 0.2759 0.1227 0.0545 0.1017 31 0.5904 0.2811 0.1437 0.0614 0.0288 -0.0004 0 0 0 0 0 0 0 Table 3 shows the results of polyclonal antibody after subscription of blank Result of week 2 practical 1 2 3 4 A 0.6084 0.5426 0.4306 0.419 B 0.5699 0.4589 0.2425 0.2505 C 0.5602 0.4504 D 0.5085 0.4093 E 0.4238 0.3164 F 0.3004 0.2355 G 0.1997 0.1794 H 0.1242 0.1093 Table 4 shows the result of rabbit IgG absorbance and two unknown sample Concentration IgG (ng/ml) 1 2 mean mean- blank sample sample mean mean- blank 2000 0.6084 0.5426 0.5755 0.45875 X=0.4306 X=0.419 0.4248 0.308 1000 0.5699 0.4589 0.5144 0.39765 Y=0.2425 Y=0.2505 0.2465 0.1297 500 0.5602 0.4504 0.5053 0.38855 250 0.5085 0.4093 0.4589 0.34215 125 0.4238 0.3164 0.3701 0.25335 62 0.3004 0.2355 0.26795 0.1512 31 0.1997 0.1794 0.18955 0.0728 0 0.1242 0.1093 0.11675 0 Table 5 shows the steps of rabbit IgG and two unknown sample, mean then subscription of blank to make calibration curve and equation to get the concentration of sample X and X Abbreviation RIA Radioimmunoassay EIA Enzyme immunoassay FIA Fluorescent immunoassay ELISA Enzyme-linked immunosorbent assay PBS Phosphate buffer saline BSA Bovine serum albumin TMB Tetramethyl benzidine HRP Horseradish peroxidase 1M HCL 1 molar of Hydrochloric acid Log Logarithm Y Absorbance IgG immunoglobulin G X Concentration Result ignore

Lion in Winter Play review :: essays research papers

â€Å"The Lion in Winter† was performed on Saturday November 22nd was not as good as I thought it was going to be. The set was a great design and looked as it should for the time period. However, I didn’t like how scenes were changed. The set should have been designed to encompass more aspects of the scene structure. There was a lot of unnecessary movement on stage when there shouldn’t have been. Stage hands should not be seen or heard. Maybe the curtains could have been drawn for some of their work. The audience shouldn’t be made to watch the stage hands reset the stage right after an intermission. I thought it was handled very unprofessionally.   Ã‚  Ã‚  Ã‚  Ã‚  Many times throughout the course of the play I heard pretty much all of the characters stumble over lines. This was not very professional either. All of the characters except for the queen were not into the play they were putting on. It showed thru really badly. Many times during dialogue there were pauses between sentences as if no one was aware of what the next line was going to be. Had this been a Broadway play it wouldn’t have made it to the second night of production. The only people that were actually prepared for production were the two characters that weren’t students. The cueing is what make or breaks a play and it broke this one all the pieces right in front of everyone’s eyes. The people who went to the show I was at were not laughing when they should be, weren’t moping with the characters like they should have been. I felt no life in this performance. It really was a sad dissertation of what I had been told and led to believ e was going to be great. Lion in Winter Play review :: essays research papers â€Å"The Lion in Winter† was performed on Saturday November 22nd was not as good as I thought it was going to be. The set was a great design and looked as it should for the time period. However, I didn’t like how scenes were changed. The set should have been designed to encompass more aspects of the scene structure. There was a lot of unnecessary movement on stage when there shouldn’t have been. Stage hands should not be seen or heard. Maybe the curtains could have been drawn for some of their work. The audience shouldn’t be made to watch the stage hands reset the stage right after an intermission. I thought it was handled very unprofessionally.   Ã‚  Ã‚  Ã‚  Ã‚  Many times throughout the course of the play I heard pretty much all of the characters stumble over lines. This was not very professional either. All of the characters except for the queen were not into the play they were putting on. It showed thru really badly. Many times during dialogue there were pauses between sentences as if no one was aware of what the next line was going to be. Had this been a Broadway play it wouldn’t have made it to the second night of production. The only people that were actually prepared for production were the two characters that weren’t students. The cueing is what make or breaks a play and it broke this one all the pieces right in front of everyone’s eyes. The people who went to the show I was at were not laughing when they should be, weren’t moping with the characters like they should have been. I felt no life in this performance. It really was a sad dissertation of what I had been told and led to believ e was going to be great.

Brave New World By Alduos Huxley :: Free Essay Writer

Brave New World By Alduos Huxley Brave New World by Aldous Huxley is a book full of meaning and purpose. Even though it was written in 1932 and wasn’t completely accepted at the time, today people accept it as a work of written genius. The book starts off as telling of mans destiny in the future. It is so far into the future that it isn’t even on the time scale of BC or AD, it is AF. There are no parents, no relatives, and no family history. Children are test tube babies in which they are grown and â€Å"born† in a building and live there and learn until they are old enough to leave and live their own lives. The babies are categorized as Alpha’s, Beta’s, Gamma’s, Delta’s and Epsilons. Alpha’s and Beta’s are high class while Gamma’s Delta’s and Epsilon’s are low class and work at factory like places. The people work to make the babies and to make the society a happy place to live in. The only culture that lives on is English; dead languages are everything else like French and Polish. The only society that still lived on was the Indians. Huxley seemed to make his novel a little debauched. The people in the town took a drug called soma and had sex casually. There were even meetings where a group of people would get together, take soma, say rhymes like â€Å"orgy-porgy† and have an orgy. For the time period this book was written, casual sex was not something accepted by the majority of people. This is one reason why the book wasn’t liked. The way Huxley started with the characters Bernard and Lenina made it seem like something big was going to happen to them at the end. Bernard was kind of a rebel from his society by not wanting to take soma and have sex with a different girl every night. And Lenina was the girl of Bernard’s fancy, who he wanted to be with, but Lenina agreed with the consumption of soma and having sex all the time. When Bernard and Lenina went to New Mexico for a holiday, they met some Indians or â€Å"savages†. But for some odd reason they met two English or â€Å"civilized† people there. A woman was left there by a man who was now England’s Director, and she got pregnant with his baby, John, who had a tormented childhood from the Indian children for his race and his mother who still lived with the civilized idea of casual sex, which the Indians did not.

The Elixir of Love

I watched Leisure damper (also called The Elixir of Love), a comic opera performed in two acts, created by the Italian composer Cottage Downsized in the nineteenth century. It was the most often performed opera in Italy between 1838 and 1848. The play showed the love triangle between the main characters. Merino, a young villager, was in love with the beautiful farm owner Adding. Unfortunately, he never knew how to get her attention; she was very indifferent and, apparently, she did not care about love at all.Hopeless, Merino .NET to see the traveling quack doctor, Dulcimers, to ask him for a love potion that could make Adding fall for him. Things got worse when Sergeant Belcher arrived in town. He felt immediately attracted to Adding as well and asked the lady to marry him. Adding, to get revenge on Merino, accepted the proposal. Thenceforth, Merino had to try his best to clarify the situation and seduce the beautiful lady. To my eyes, the whole play was completely addictive and hear t-wringing. Furthermore, I felt really involved with the characters and the ending was very satisfying.The setting was extraordinary. Although It was always the same background, the light fixture and additional ornamentation made it look flawless all the time. There were also some visual effects that helped a lot to create appropriate situations, for Instance, the different times of the day. The costumes of the cast were totally accurate with the era that the play was trying to represent. They presented beautiful dresses for the ladies and elegant or humble outfits for the men, depending on their characters, of course. As for the music and the acting, I must admit I was massively Impressed.I had never watched an opera before, but I had seen lots of plays. Nevertheless, this has been my favorite so far. The music was outstanding. Every time one of the cast members started to sing, I felt out of this world. They had an Impressive talent and vocal ability, and the orchestra also did an amazing Job. Moreover, the acting was superb. They were all really charming and committed. The cast had the power to bring me from laughter to tears In Just a second. Allele's damper Is definitely an opera that I would go watch again If possible. It was terrific!

First Five Years/ Descriptive Essay Essay

I was born on the island of Sao Miguel, Azores which is part of Portugal. Sao Miguel is also known as â€Å"Green Island† due to its lush meadow landscape, rain forests and waterfalls. I was blessed in being born to Eduardo & Olga Pereira. The story begins with my father; he entered the military â€Å"Army† as soon as he was of legal age. My mother was a live-in nanny for a wealthy family who lived in a white house on the hillside overlooking the city. She has told me how she loved being a nanny for the little boy who was called Roberto Reis. She often talks about the family because she has found memories of caring for the little boy. Also, being of a young age of 15 the family cared for her as one of their own children. This was the beginning to how my parents met. According to dad he was walking one day down a cobblestone road and spotted my mother on the grey iron veranda†¦ she was wearing a white dress, slim with long dark hair and very pretty and holding the l ittle boy. He tried talking to my mom at the time but she said she couldn’t be bothered. At least that’s how she tells the story. Nonetheless he started walking by every day at high noon in hopes of talking and courting my mom from the veranda. The family that she worked for had strict family values when it came to respecting someone’s daughter. The father figure in the house†¦said to my dad, Olga lives underneath our roof so I’m held accountable for her safety and well-being. She is a part of our family so I expect only honorable intentions. My dad respectfully informed him, his interest were for a long-term commitment. A couple of years later my father and mother got married and I came along shortly a year later. When I turned 2yrs of age my mother told me the story of how my maternal grandmother told her that she would not watch me again because I’d given her a heart attack. It seems that I was fond of kittens so I apparently saw one outside and decided to follow it right to a 2ft rock wall which I climbed and began crawling towards the orange colored kitten. When my grandmother finally noticed me she’d just about had a heart attack because behind that wall was 25 foot drop over rocks into grapevines. She slowly and carefully walked slowly calling my name to come to her and get off the wall. As she inched her way close enough she grabbed me and held so tight. She was just so  thankful that nothing happened to me. So as I stated earlier no more quality time at vovo’s house for me without my mom being present and accounted for. Once I reached 2  ½ years of age is when my parents and I migrated to the United States of America in April 1971. The â€Å"American Dream† is what my parents were in pursuit of which led them to the City of Fall My father’s sister lived here with her husband and children. It was very early on as to the goals my parents had set for themselves. My dad was a carpenter and built sailing yachts, and mom worked in a mill as a sewing machine operator. It was all about working hard and long hours and saving money to buy a car and then a house. Both parents didn’t go to college in Portugal but had enough education to reach the goals they set for themselves. I remember we always had everything we needed at least that’s what they showed growing up. The culture Roman Catholic was an enormous part of life. Going to church weekly and participation in church functions â€Å"The Feast of Our Lady of Health† were celebrated every August. The church held Saturday night dinner dances the men wore casual bell bottom pants and women wore white gogo boots†¦..it was all for raising money for the church often usually once a month as well as the New Year Party which brought the parishioner’s together to celebrate the new coming year. I was enrolled at SS Peter and Paul School. Learning Religion was very important to my parents. One memory in the 2nd grades I remember was the annual Halloween party at school. The PTA would decorate the hall with black and orange streamers and setup a witch’s cauldron with smoke coming out of it and we would bob for apples and get tricks or treat bags. It was such fun. I also enjoyed when we did fundraisers at school I liked bringing home the huge boxed kits full of items to show and sell on tracking sheet. I would constantly take items out and repack them I remember enjoying to the touch, the feel of things and even the smell of these little blue car erasers. I was so proud of selling my items I was known as the little brown haired girl who was assertive in selling all the different trinkets. I remember long ago how my parent’s friends would many times say to me â€Å"How time passes so quickly with respect to growth and time â€Å" I didn’t realize  how many fond memories I had of my early childhood until today. As I finish this essay the sun is shining bright yellow and my background music is calm meditation.

PSI Autos Glossary Definition

The acronym PSI stands for Pounds per Square Inch, and is the common unit of measurement for pressure. It can be understood as the amount of force that is exerted on an area of one square inch. Normal atmospheric pressure at sea level is 14.7 PSI. Pronunciation: Pronounce each letter individually:Â  P - S - I. Example: Normal tire pressure is usually about 32 PSI.

Find the Anode and Cathode of a Galvanic Cell

​Anodes and cathodes are the endpoints or terminals of a device that produces electrical current. Electrical current runs from the positively charged terminal to the negatively charged terminal. The cathode is the terminal that attracts cations, or positive ions. To attract the cations, the terminal must be negatively charged. Electrical current is the amount of charge that passes a fixed point per unit time. The direction of the current flow is the direction in which a positive charge flows. Electrons are negatively charged and move in the opposite direction of the current. In a galvanic cell, the current is produced by connecting an oxidation reaction to a reduction reaction in an electrolyte solution. Oxidation and reduction reactions or redox reactions are chemical reactions involving a transfer of electrons from one atom in the reaction to another. When two different oxidation or reduction reactions are connected electrically, a current is formed. The direction depends on the type of reaction taking place at the terminal.Reduction reactions involve the gain of electrons. Electrons are needed to fuel the reaction and pull these electrons from the electrolyte. Since electrons are attracted to the reduction site and current flows opposite the flow of electrons, current flows away from the reduction site. Since current flows from the cathode to the anode, the reduction site is the cathode.Oxidation reactions involve the loss of electrons. As the reaction progresses, the oxidation terminal loses electrons to the electrolyte. The negative charge moves awa y from the oxidation site. The positive current moves towards the oxidation site, against the flow of electrons. Since current flows to the anode, the oxidation site is the anode of the cell. Keeping Anode and Cathode Straight On a commercial battery, the anode and cathode are clearly marked (- for anode and for cathode). Sometimes only the () terminal is marked. On a battery, the bumpy side is () and the smooth side is (-). If youre setting up a galvanic cell, youll need to keep the redox reaction in mind to identify the electrodes. Anode: positively charged terminal - oxidation reactionCathode: negatively charged terminal - reduction reactionThere are a couple mnemonics that can help you remember the details.To remember the charge: Caions are attracted to the Cahode (the t is a plus sign)To remember which reaction occurs at which terminal: An Ox and Red Cat - Anode Oxidation, Reduction Cathode Remember, the concept of electrical current was defined back before scientists understood the nature of positive and negative charges, so it was set up for the direction a () charge would move. In metals and other conductive materials, its actually the electrons or (-) charges that move. You can think of it as holes of positive charge. In an electrochemical cell, its just as likely cations will move as anions (in fact, both are probably moving at the same time).